OUTLINE OF PROTOCOL 021

 

A PHASE I SAFETY AND IMMUNOGENICITY TRIAL OF UBI® HIV LIPOPEPTIDE IMMUNOTHERAPEUTIC IN HIV-1 SERONEGATIVE HUMAN SUBJECTS

 

Subjects: Healthy, HIV-1 uninfected (seronegative) volunteers.

Schema:

Treatment Schedule



Group



Dose

Accrual*



Route

Week 0

Day 0

Week 4

Day 28

Week 8

Day 56

Week 12

Day 84

Week 36-52

I

70 µg

12

s.c.

X

X

X

X

X

Placebo

3

s.c.

X

X

X

X

X

II

350 µg

12

s.c.

X

X

X

X

X

Placebo

3

s.c.

X

X

X

X

X

Total n = 30

* Accrual was closed at 29 enrollees
** Study extension

ACCRUAL, IMMUNIZATIONS AND FOLLOW-UP COMPLETED

 

Product Description: The HIV-1 gag lipopeptide, P3C541b, is a linear peptide representing a sequence of the HIV gag protein conjugated to a lipid moiety and solubilized for parenteral administration. [United Biomedical, Inc.]

Time Period: First volunteer enrolled on 01/04/95; and the last on 09/26/95. Follow-up extended to 24 weeks (6 months) after last immunization.

Clinical Sites: Johns Hopkins University, Vanderbilt University and University of Washington

Study Chair: David Schwartz, Johns Hopkins University

 

INCLUSION CRITERIA

Standard inclusion criteria, with the addition of:

 

EXCLUSION CRITERIA

Standard exclusion criteria.

 

STUDY GOALS

The aims of this Phase I study are to evaluate the safety and immunogenicity of an HIV synthetic lipopeptide vaccine component - the following specific primary questions will be addressed: a)Are there any adverse clinical reactions to the candidate vaccine (i.e., local or systemic reactions); is there any laboratory evidence of vaccine-induced toxicity?; b) Does the candidate vaccine stimulate CD8+ and/or CD4+ cytotoxic T-cell responses?; c) Is there a dose-response detectable between 70 µg and 350 µg for either toxicity or immunogenicity?

Study Extension: Addition of a fifth dose to obtain additional information about the safety and immunogenicity of the vaccine; specifically, do antibody levels and/or CTL responses increase after a rest period between immunizations?