Z Moodie, Y Huang, L Gu, J Hural, and S Self. Statistical positivity criteria for the analysis of ELISpot assay data in HIV-1 vaccine trials. J. of Imm. Meth. 315: 121-132, 2006.
Z Moodie*, L Price*, C Gouttefangeas, A Mander, S Janetzki, M Lower, MJ Welters, C Ottensmeier, SH van der Burg, CM Britten. Response definition criteria for ELISpot assays revisited. Cancer Immunol Immunother, 59(10):1489-501, 2010.
*shared first authorship
Z Moodie, L Price, S Janetzki, CM Britten. Response determination criteria for ELISPOT: toward a standard that can be applied across laboratories. Methods Mol Biol. 792:185-96, 2012.
The statistical software R is a freeware, and the installation file can be downloaded here.