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runDFR Web Tool

This web tool determines positivity calls for ELISpot data according to the DFR(eq) and DFR(2x) methods described in:

Z Moodie, Y Huang, L Gu, J Hural, and S Self. Statistical positivity criteria for the analysis of ELISpot assay data in HIV-1 vaccine trials. J. of Imm. Meth. 315: 121-132, 2006.

Z Moodie*, L Price*, C Gouttefangeas, A Mander, S Janetzki, M Lower, MJ Welters, C Ottensmeier, SH van der Burg, CM Britten. Response definition criteria for ELISpot assays revisited. Cancer Immunol Immunother, 59(10):1489-501, 2010. *shared first authorship

Z Moodie, L Price, S Janetzki, CM Britten. Response determination criteria for ELISPOT: toward a standard that can be applied across laboratories. Methods Mol Biol. 792:185-96, 2012.

DISCLAIMER: The information is provided "as is". There are no warranties, expressed or implied, as to merchantability or fitness for a particular purpose regarding the accuracy of the materials or code herein.
Number of antigens:
Number of experimental wells:
Number of control wells:
Name of negative control:
Submit your .csv data file here (view an example):
NOTE: File must be in the exact format with the same column order: id, day, antigen (with negative control name exactly as entered above), well-level responses.

The R code that implements the DFR(eq) and DFR(2x) methods is available for download as elsdfreq.R and elsdfr2x.R, respectively.

The statistical software R is a freeware, and the installation file can be downloaded here.